2014-01-01

Application of PCR-SSP method for HLA-B*27 identification as an auxiliary tool for diagnosis of ankylosing spondylitis specificity(27-32). The HLA-B*27:05 and the HLA-B27:02* are the most common subtypes, 90% and 5%-10%, respectively, in Caucasians(33); the HLA-B*27:04 is the predominant subtype among Chinese and Japanese (34) and the HLA-B*27:04,

2014-01-01 · Most molecular HLA typing methods are based on the group-specific amplification by PCR where the PCR-SSP technique is widely used to detect HLA-B* 27 [20]. In this study, we developed an in-house PCR-SSP test which amplifies all the HLA-B* 27 alleles (27:01-27:73) except B* 27:18 and B* 27:23, which have not been reported from Asian population. HLA typing by sequence-specific oligonucleotides probes (PCR-SSOP) Example of hybridization specificity with SSO probes. The combination of PCR technology and hybridization with sequence-specific oligonucleotide probes was first applied to HLA class II typing because of the limitations of DR serology and of the better knowledge of allelic polymorphism at DR/DQ loci. 24 PCR reactions; 24 typings per kit 主页 > 临床诊断 > Products > 免疫血液学 > Seraclone/TANGO optimo 系统/HLA > 移植诊断 > HLA DNA > SSP > HLA-A Kit de diagnóstico cualitativo in vitro por PCR-SSP para el tipaje de baja y alta resolución mediante ADN de los alelos HLA de clase I y clase II. Solicitar HLA-DR typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours: an alternative to serological DR typing in clinical practice including Aplicação da metodologia de PCR-SSP na identificação de HLA-B*27 como Standardize the single specific primer-polymerase chain reaction (PCR-SSP) los genes HLA (exón 2 en los genes HLA-DR, -.

Pcr ssp hla

Sequence-specific amplification (SSP) is simply a form of polymerase chain reaction (PCR) which involves designing one or both primers so that they will or will not allow amplification (the 3'-mismatch principle). Its origins are probably legion, i.e. many people probably thought of it at the same time. Olerup SSP HLA Typing Kits are qualitative in vitro diagnostic kits for the DNA typing of HLA Class I and HLA Class II alleles. The products are used by trained professionals in medical settings for the purpose of determining HLA phenotype.

T1 - A new PCR-SSP method for HLA DR-DQ risk assessment for celiac disease. AU - Lavant, Ewa H. AU - Agardh, Daniel.

Studien syftade till att bedöma HLA-kompatibilitet, HLA-antikroppar och korsat i Vävnadstypning för HLA utfördes av en SSP-PCR (sekvensspecifik primer).

• Basado en HLA-A2 (s). HLA-A *02:01-05. HLA-A *02:01.

There are two basic techniques for HLA-typing: Serological HLA-typing (antibody-based typing). Old school! Genomic HLA-typing (PCR-based). PCR-SSP

Immunologi SLL51219. Kvant CD4, CD8 flow. 175.

简介. 采用SSP进行 PCR 扩增后，相应的PCR产物是否出现，是HLA分型结果的判断的依据，相应SSP扩增产物出现，表示基因组中存在与特异性引物（即SSP）互补结合的 DNA 序列，即样本为该SSP结合的HLA 基因阳性。. 当某种SSP引物的扩增管中未出现相应的PCR产物时，应注意观察内参照引物是否出现PCR产物。. 如果该扩增管的内参照引物出现PCR产物，而无相应SSP扩增产物，说明样本 HLA-DR typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours: An alternative to serological DR typing in clinical practice including donor-recipient matching in cadaveric PCR-SSP HLA分型技术 PCR-SSP（sequence specific primer）分型技术是根据编码各种HLA抗原的等位基因的碱基排列顺序，设计并合成与之互补的寡核苷酸作为PCR引物，样本中HLA等位基因能够用相应的引物（即SSP）进行扩增。 Genomic HLA class I typing, which uses polymerase-chain reaction with sequence-specific primers (PCR-SSP), gave discrepant results in 3-24% of the patients, compared to serological typings. hla class i and ii typing kits (ssp-pcr) hla-disease association kits based on ssp hla-ready gene a, -b, -c, -dr, -dq and -dpb1 hla-ready gene b27 – ankylosing Currently, PCR-SSP, PCR-SSOP, and PCR-SBT are the most widely used HLA typing methods allow a medium- or high-resolution throughput.
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T1 - A new PCR-SSP method for HLA DR-DQ risk assessment for celiac disease.

Most molecular HLA typing methods are based on the group-specific amplification by PCR where the PCR-SSP technique is widely used to detect HLA-B* 27 .
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HKQ, HKR, HKS, HKT, HKU, HKV, HKW, HKX, HKY, HKZ, HLA, HLB, HLC PCK, PCL, PCM, PCN, PCO, PCP, PCQ, PCR, PCS, PCT, PCU, PCV, PCW SSM, SSN, SSO, SSP, SSQ, SSR, SSS, SST, SSU, SSV, SSW, SSX

T1 - A new PCR-SSP method for HLA DR-DQ risk assessment for celiac disease. AU - Lavant, Ewa H. AU - Agardh, Daniel. AU - Ramelius, Anita BACKGROUND: Susceptibility to celiac disease is essentially restricted to carriers of specific HLA DQA1 and DQB1 alleles. We have developed a semi-automated sequence specific primer (SSP) PCR method for clinical HLA typing and compared the test results with those from a commercial method. We have developed a semi-automated sequence specific primer (SSP) PCR method for clinical HLA typing and compared the test results with those from a commercial method. Methods: Primers for each DQA1 and DQB1 allele group were included in our PCR-SSP reaction to allow differentiation of homozygous from heterozygous carriers of risk alleles.